dissertação
Armazenamento de adultos de Orius insidiosus (SAY, 1832) (Hemiptera: Anthocoridae) em baixa temperatura
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UNIVERSIDADE FEDERAL DE LAVRAS
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A habilidade em armazenar inimigos naturais é um fator chave para o controle biológico aumentativo, permitindo maior flexibilidade na criação massal, na embalagem e no transporte dos insetos benéficos. Este trabalho teve como objetivo avaliar o efeito do armazenamento sob baixa temperatura na sobrevivência e nos parâmetros reprodutivos de Orius insidiosus (Say, 1832), bem como na sua longevidade. Ninfas deste predador foram individualizadas e após a emergência dos adultos, fêmeas virgens, fêmeas acasaladas com machos não armazenados, fêmeas acasaladas com machos armazenados e machos virgens foram submetidos aos períodos de 5, 10, 15 e 20 dias de armazenamento sem alimento em câmara climática a 8±2°C, UR 70±10% e escotofase constante. Como tratamento controle, fêmeas acasaladas e machos virgens foram mantidos a 25°C, UR 70±10% e fotofase de 12 horas, sendo alimentados ad libitum. Após cada período de armazenamento, foram reaclimatados e avaliada a sobrevivência de 200 fêmeas virgens, 200 fêmeas acasaladas e 50 machos virgens. Foram amostrados 10 machos virgens, 20 fêmeas acasaladas antes e 20 fêmeas acasaladas após o armazenamento, visando avaliar os parâmetros reprodutivos e a longevidade. O número de adultos vivos de O. insidiosus diminuiu com o aumento do período de armazenamento. A sobrevivência das fêmeas (92,0; 86,0; 85,3 e 75,0%) foi maior do que a de machos (62,0; 62,0; 58,0 e 22,0%, respectivamente) em todos os períodos de armazenamento testados. O período de pré-oviposição das fêmeas aumentou após maiores períodos de armazenamento, de 2,2±0,20; 2,1±0,10; 2,2±0,29 e 2,1±0,28 dias, respectivamente após 5 dias de armazenamento para 4,6±0,56; 4,4±0,64; 3,4±0,40 e 3,1±0,23 dias, respectivamente, após 20 dias. O período de oviposição e a fecundidade diária das fêmeas submetidas ao armazenamento diminuíram com o aumento da exposição ao frio. Após os períodos de 5, 10, 15 dias de armazenamento as fêmeas acasaladas com machos não armazenados e posteriormente armazenadas tiveram uma maior fecundidade total (53,0±11,22; 54,9±8,12 e 36,2±9,34 ovos, respectivamente) em relação às fêmeas submetidas aos demais tratamentos. A maior viabilidade de ovos foi observada naqueles produzidos por fêmeas acasaladas após o armazenamento. A longevidade de fêmeas foi maior do que a de machos em todos os períodos de armazenamento testados. Estes resultados podem contribuir para o aprimoramento da técnica de armazenamento que menos comprometa a qualidade de O. insidiosus.
The ability to store natural enemies is a key factor for augmentative biological control, allowing greater flexibility in mass production, packaging and transportation of beneficial insects. This study aimed to evaluate the effect of storage under low temperature on survival and reproductive parameters of Orius insidiosus (Say, 1832), as well as its longevity. Nymphs of this predator were individualized and after adult emergence, virgin females, females mated with males not stored, females mated with males stored and virgin males were exposed to periods of 5, 10, 15 and 20 days storage in a climatic chamber to 8 ± 2 °C, RH 70 ± 10% and constant scotophase and without food. As control treatment, females mated and virgin males were maintained at 25 °C, RH 70 ± 10% and photophase of 12 hours, and food ad libitum. After each storage period were acclimated and the survival of 200 virgin females, 200 males and 50 females with virgins were evaluated. We sampled 10 virgin males, 20 females with 20 mated females before and after storage, to evaluate the reproductive parameters and longevity. The number of adults living O. insidiosus decreased with increasing storage period. The survival of females (92.0, 86.0, 85.3 and 75.0%) was higher than that of males (62.0, 62.0, 58.0 and 22.0%, respectively) all storage periods tested. The pre-oviposition of females increased after 5 days of storage, 2.2 ± 0.20, 0.10 ± 2.1, 2.2 ± 0.29 and 2.1 ± 0.28 days, to 4.6 ± 0.56, 0.64 ± 4.4, 3.4 and 3.1 ± 0.40 ± 0.23 days, respectively, after 20 days. Oviposition period and fecundity of the female exposed to daily storage decreased with increased exposure to cold. After periods of 5, 10, 15 days of storage the females mated with males not stored and subsequently stored had a higher total fertility rate (53.0 ± 11.22, 54.9 ± 8.12 and 36.2 ± 9, 34 eggs, respectively) compared to females exposed to other treatments. The largest egg viability was observed in those produced by females mated after storage. The longevity of females was higher than that of males in all storage periods tested. These results can contribute to the improvement of storage technique that conserve the quality of O. insidiosus.
The ability to store natural enemies is a key factor for augmentative biological control, allowing greater flexibility in mass production, packaging and transportation of beneficial insects. This study aimed to evaluate the effect of storage under low temperature on survival and reproductive parameters of Orius insidiosus (Say, 1832), as well as its longevity. Nymphs of this predator were individualized and after adult emergence, virgin females, females mated with males not stored, females mated with males stored and virgin males were exposed to periods of 5, 10, 15 and 20 days storage in a climatic chamber to 8 ± 2 °C, RH 70 ± 10% and constant scotophase and without food. As control treatment, females mated and virgin males were maintained at 25 °C, RH 70 ± 10% and photophase of 12 hours, and food ad libitum. After each storage period were acclimated and the survival of 200 virgin females, 200 males and 50 females with virgins were evaluated. We sampled 10 virgin males, 20 females with 20 mated females before and after storage, to evaluate the reproductive parameters and longevity. The number of adults living O. insidiosus decreased with increasing storage period. The survival of females (92.0, 86.0, 85.3 and 75.0%) was higher than that of males (62.0, 62.0, 58.0 and 22.0%, respectively) all storage periods tested. The pre-oviposition of females increased after 5 days of storage, 2.2 ± 0.20, 0.10 ± 2.1, 2.2 ± 0.29 and 2.1 ± 0.28 days, to 4.6 ± 0.56, 0.64 ± 4.4, 3.4 and 3.1 ± 0.40 ± 0.23 days, respectively, after 20 days. Oviposition period and fecundity of the female exposed to daily storage decreased with increased exposure to cold. After periods of 5, 10, 15 days of storage the females mated with males not stored and subsequently stored had a higher total fertility rate (53.0 ± 11.22, 54.9 ± 8.12 and 36.2 ± 9, 34 eggs, respectively) compared to females exposed to other treatments. The largest egg viability was observed in those produced by females mated after storage. The longevity of females was higher than that of males in all storage periods tested. These results can contribute to the improvement of storage technique that conserve the quality of O. insidiosus.
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LOPES, L. R. Armazenamento de adultos de Orius insidiosus (SAY, 1832) (Hemiptera: Anthocoridae) em baixa temperatura. 2011. 52 p. Dissertação (Mestrado em Agronomia/Entomologia)-Universidade Federal de Lavras, Lavras, 2011.