Ação inibitória de extratos de plantas sobre lipase pancreática com ênfase em Baccharis trimera (less.) DC

Abstract

Obesity is a problem of world order, and represents a serious health problem for the individual, generates large expenditures for the country. The search for anti-obesity agents is increasingly growing, and the inhibition of lipase is a promising alternative, to act exclusively in the small intestine. This study investigated the potential inhibition of lipase by methanol extracts of medicinal plants with emphasis on the broom. Were prepared methanol extracts of 19 plants, of these, 8 had an inhibitory activity on the lipase. Extract shrub was selected for continued testing. The aqueous extract and tea shrub, showed no inhibitory activity, the ethanol inhibited 16% and 78% methanol extract. The methanol extract was subjected to a cleaning solvent with different polarity and methanol fraction inhibited the lipase in 72%. As the tea shrub is commonly used as a tool in anti-obesity treatment, and it did not show inhibitory activity on the lipase, then evaluated the inhibition of tea and the methanol extract on the activity of glycosidases (α - glucosidase, α - amylase, β - glucosidase). The α-amylase was not inhibited by both extracts, the enzyme α-glucosidase was inhibited by both extracts in the same proportion (46.9 ± 0.1), the β-glucosidase was inhibited by 73% methanol extract and 65 % for tea. We investigated then the slimming effect and hypolipidemic methanol extract of broom (EMC) in mice. We used 21 rats were randomly divided into three groups with 7 animals per group. The administration of the extract was performed orally for 35 days. Group A (control) received 1 mL of water / day, group B received a dose of 0.003 g / day (dose 1) and group C at a dose of 0.1 g / day (dose 2). Assessments were made weekly levels of cholesterol and triglycerides. The treated mice suffered significant weight reduction compared to the control group did not differ between doses. Despite showing an increase in cholesterol levels caused by induction diet rats given doses of EMC have risen less characterizing a hypocholesterolemic effect. LDL levels were lower in the group receiving the dose of 0.1 g / day. The content of total lipids in the liver was higher in the control group. In the liver of some animals we observed a slight vacuolization, not significant. In conclusion the results suggest that EMC has weight loss and hypolipidemic action, possibly by the inhibitory action of pancreatic lipase. Given the absence of mortality and toxic effects are strong evidence that the EMC can be used to aid the treatment of obesity and its side effects, as well as in subjects with hypercholesterolemia.