Isolamento e cultivo de células para estudos em nutrição animal

Abstract

The cellular and molecular biology studies structures and events very thorough, and therefore, depends on the improvement of tools and techniques of research. The improvement of methods for the separation of cellular organelles, as well as for in vitro studies of molecules and their functions are benefits to research related to the physiology and metabolism, these skills are essential to progress in animal nutrition. Cells taken from animals can be kept viable for study for some time. To this must be preserved amid isotonic, thus avoiding change in its volume. When you want to study living cells for a longer time, the use of methods of cultivation can be an interesting alternative. The isolation of hepatocytes in fish is a technique widely used in tests of metabolism, as a way to evaluate the action of enzyme complexes, whose determination of its activity depends, for example, marking with radioactive compounds. Thus, it is common the isolation of hepatocytes and marking of fish with radioisotopes for assessing the activity of enzymes of the biosynthesis of fatty acids, such as desaturase and elongases, which require cell culture techniques for further incubation with 14C.