Changes in hydraulic architecture across a water availability gradient for two contrasting commercial Eucalyptus clones

Abstract

The increase in global temperature results in changes in rainfall patterns and can cause structural and functional changes in trees. Each plant is forced to face hydraulic safety limits and adopt a behavior to regulate its water status. The objective of this work was to evaluate the changes in the hydraulic architecture of the xylem of commercial eucalyptus clones in order to distinguish the hydraulic adjustments in a wide gradient of water availability. Eucalyptus urophylla (A1) and E. grandis × E. camaldulensis (C3) clones at six years old at 4 sites in Brazil distributed in a rainfall gradient and in two water conditions were analyzed: control (100% of total local rainfall) and rainfall reduction (RR). Xylem traits were evaluated at annual intervals and averaged over the entire growth period of the seven trees sampled at each site and water condition. The sites influenced the clones, with the greatest effect being the rainfall reduction treatment in C3 on the vessel wall diameter, area and thickness, double wall thickness, conduction area, vessel composition index, theoretical and potential hydraulic conductivities, vessel implosion resistance and vulnerability. The rainfall reduction provided an increase of 12% in vessel density, 8% in the double vessel wall, and 27% in theoretical hydraulic conductivity in the drought-tolerant C3 clone. The vessel wall thickness in the A1 clone was more responsive with a 3% increase in rainfall reduction, with an effect on double-wall thickness and vessel implosion resistance. Other hydraulic adjustment strategies were observed, such as the highest occurrence of tylose and a tradeoff between vessel diameter and frequency for the C3 clone. The greater variation in wood density was explained by variations in the lumen area of the xylem vessels and by vessel implosion resistance.