Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.

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dc.creatorKoblitz, Maria Gabriela Bello
dc.creatorPastore, Gláucia Maria
dc.date2006-06-01
dc.date.accessioned2015-04-30T13:33:47Z
dc.date.available2015-04-30T13:33:47Z
dc.date.issued2015-04-30
dc.description.resumoThe present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+.
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dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000300016
dc.identifier.citationKOBLITZ, M. G. B.; PASTORE, G. M. Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp. Ciência e Agrotecnologia, Lavras, v. 30, n. 3 , p. 494-502, maio 2006.
dc.identifier.urihttps://repositorio.ufla.br/handle/1/6226
dc.publisherEditora da Universidade Federal de Lavras
dc.sourceCiência e Agrotecnologia v.30 n.3 2006
dc.subjectLipase
dc.subjectRhizopus sp.
dc.subjectPurification
dc.subjectBiochemical characterization
dc.titlePurification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.
dc.typejournal article

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