dissertação
Avaliação de nematóides entomopatogênicos (Rhabditida) para o controle da mosca-das-frutas Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)
Carregando...
Notas
Data
Autores
Orientadores
Editores
Coorientadores
Membros de banca
Título da Revista
ISSN da Revista
Título de Volume
Editor
UNIVERSIDADE FEDERAL DE LAVRAS
Faculdade, Instituto ou Escola
Departamento
Programa de Pós-Graduação
DEN - Programa de Pós-graduação
Agência de fomento
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Tipo de impacto
Áreas Temáticas da Extenção
Objetivos de Desenvolvimento Sustentável
Dados abertos
Resumo
A mosca-das-frutas é considerada uma das principais pragas da fruticultura mundial, acarretando perdas significativas para este setor. Este inseto passa uma fase de sua vida no solo, sendo alvo em potencial para nematóides entomopatogênicos. Assim, este trabalho teve como objetivos avaliar a eficiência de diferentes isolados de nematóides entomopatogênicos contra larvas e pupas de Ceratitis capitata (Diptera: Tephritidae), bem como avaliar a influência da temperatura e umidade do solo sobre a eficiência deste entomopatógeno. Foi avaliada a patogenicidade de diferentes isolados de Steinernema e Heterorhabditis sobre larvas e pupas de C. capitata. Cada tratamento teve quatro repetições, com 20 indivíduos cada, acondicionados em placas de Petri, sobre a qual foi inoculado 1 mL de suspensão com 200 JI/inseto. Os isolados mais virulentos para larvas foram selecionados e avaliados nas concentrações que variaram de 50 a 400JI/inseto. Foi avaliada, ainda, a eficiência de S. carpocapsae e Heterorhabditis sp. RSC01 quando aplicados de forma isolada ou combinada, o efeito da aplicação do nematóide em diferentes períodos de infestação do solo com larvas, além da influência da temperatura e umidade do solo sobre a eficiência destes isolados. Estes bioensaios foram realizados em potes plásticos contendo 100 g de solo e 10 larvas de C. capitata, sendo aplicados 125JI/cm2. As avaliações foram realizadas após cinco dias. Verificou-se que todos os isolados foram patogênicos para larvas e pupas de C. capitata, sendo que S. carpocapsae e Heterorhabditis sp. RSC01 causaram mortalidade superior a 85% para a fase larval e Heterorhabditis sp. PI, Heterorhabditis sp. JPM4, H. bacteriophora HP88, S. feltiae e S. glaseri foram os mais eficientes para a fase de pupa, com a mortalidade variando entre 35 e 44%. A aplicação conjunta de S. carpocapsae e Heterorhabditis sp. RSC01 aumentou a eficiência de Heterorhabditis sp. RSC01. Independentemente do intervalo de infestação do solo com larvas, S. carpocapsae e Heterorhabditis sp. RSC01 foram eficientes contra C. capitata. A virulência dos nematóides estudados foi diretamente proporcional ao aumento da temperatura e foi baixa em condições de umidade do solo extremas.
Fruit flies is considered one of the key pest in the world fruitculture causing significant losses for this sector. This insect passes a phase of its life in the soil, being the potential target for the entomopagenic nematodes. Therefore this work aim get to evaluate the efficiency of different entomopatogenic nematod isolates against larva and pupa of Ceratitis capitata (Diptera: Tephritidae), as well as to evaluate the influence of soil temperature and wetness over the efficiency of this pathogen. It was evaluated the pathogenicity of different isolates of Steinernema e Heterorhabditis over larva and pupas of C. capitata. Each treatment had four replications, with 20 individuals each, conditioned in Petri dishes, over which it was inoculated 1 mL suspension with 200 JI/insect. The isolates more virulent for the larvae were selected and evaluated in the concentrations which ranged from 50 to 400JI/insect. It was also evaluated the efficiency of S. carpocapsae and Heterorhabditis sp. RSC01 when isolate applicated in an isolate way or combined, the effect of the nematode application in different periods of infestation of soil with larvae, besides the influence of the soil temperature and wetness over the efficiency of those isolates. These bioassays were made in plastic jars containing 100 g soil and 10 larvae of C. capitata, being applicated 125JI/cm2. The evaluations were done after five days. It was verified that all the isolates were pathogenic for the larva and pupas of C. capitata, hence that S. carpocapsae and Heterorhabditis sp. RSC01 caused mortality rate higher than 85% for the larva phase and Heterorhabditis sp. PI, Heterorhabditis sp. JPM4, H. bacteriophora HP88, S. feltiae and S. glaseri were more efficient for the pupa phase, with mortality rate ranging between 35 and 44%. The combined application of S. carpocapsae and Heterorhabditis sp. RSC01 increased the efficiency of Heterorhabditis sp. RSC01. Independently from the soil infestation interval with larvae, S. carpocapsae and Heterorhabditis sp. RSC01 were efficient against C. capitata. The virulence of studied nematodes was directly proportional to the increase of temperature and it was low in extreme soil condition of wetness.
Fruit flies is considered one of the key pest in the world fruitculture causing significant losses for this sector. This insect passes a phase of its life in the soil, being the potential target for the entomopagenic nematodes. Therefore this work aim get to evaluate the efficiency of different entomopatogenic nematod isolates against larva and pupa of Ceratitis capitata (Diptera: Tephritidae), as well as to evaluate the influence of soil temperature and wetness over the efficiency of this pathogen. It was evaluated the pathogenicity of different isolates of Steinernema e Heterorhabditis over larva and pupas of C. capitata. Each treatment had four replications, with 20 individuals each, conditioned in Petri dishes, over which it was inoculated 1 mL suspension with 200 JI/insect. The isolates more virulent for the larvae were selected and evaluated in the concentrations which ranged from 50 to 400JI/insect. It was also evaluated the efficiency of S. carpocapsae and Heterorhabditis sp. RSC01 when isolate applicated in an isolate way or combined, the effect of the nematode application in different periods of infestation of soil with larvae, besides the influence of the soil temperature and wetness over the efficiency of those isolates. These bioassays were made in plastic jars containing 100 g soil and 10 larvae of C. capitata, being applicated 125JI/cm2. The evaluations were done after five days. It was verified that all the isolates were pathogenic for the larva and pupas of C. capitata, hence that S. carpocapsae and Heterorhabditis sp. RSC01 caused mortality rate higher than 85% for the larva phase and Heterorhabditis sp. PI, Heterorhabditis sp. JPM4, H. bacteriophora HP88, S. feltiae and S. glaseri were more efficient for the pupa phase, with mortality rate ranging between 35 and 44%. The combined application of S. carpocapsae and Heterorhabditis sp. RSC01 increased the efficiency of Heterorhabditis sp. RSC01. Independently from the soil infestation interval with larvae, S. carpocapsae and Heterorhabditis sp. RSC01 were efficient against C. capitata. The virulence of studied nematodes was directly proportional to the increase of temperature and it was low in extreme soil condition of wetness.
Abstract
Descrição
Área de concentração
Entomologia Agrícola
Agência de desenvolvimento
Palavra chave
Marca
Objetivo
Procedência
Impacto da pesquisa
Resumen
Palavras-chave
ISBN
DOI
Citação
ROHDE, C. Avaliação de nematóides entomopatogênicos (Rhabditida) para o controle da mosca-das-frutas Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). 2007. 63 p. Dissertação (Mestrado Agronomia/Entomologia)-Universidade Federal de Lavras, Lavras, 2007.