Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stages

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dc.creatorTorres, Luana Ferreira
dc.creatorDiniz, Leandro Eugenio Cardamone
dc.creatorLivramento, Kalynka Gabriella do
dc.creatorFreire, Luciana Lima
dc.creatorPaiva, Luciano Vilela
dc.date.accessioned2020-06-14T02:25:20Z
dc.date.available2020-06-14T02:25:20Z
dc.date.issued2015-08
dc.description.abstractThe present study aimed to evaluate the quality of cell suspension of Coffea arabica cv. Catiguá at four different ages by morphological and gene expression analysis. For this purpose, cell suspension samples were collected at 30, 45, 60 and 75 days. Morphological analysis revealed the presence of embryogenic regions in all samples, and after 60 days, non-embryogenic regions were also identified. The genic analyses revealed that as the cells were transferred to a new medium a change in both their physical and chemical conditions was noticed that caused stress. The decrease in SERK and BBM genes expression after 75th day may also be due to the non-embryogenic regions, characterized by large, elongated and vacuolated cells that were observed in the periphery of embryogenic regions, starting from 60 days of culture.pt_BR
dc.identifier.citationTORRES, L. F. et al. Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stages. Acta Physiologiae Plantarum, [S.l.], v. 37, p. 175-181, Aug. 2015. DOI: 10.1007/s11738-015-1924-6.pt_BR
dc.identifier.urihttps://repositorio.ufla.br/handle/1/41412
dc.identifier.urihttps://link.springer.com/article/10.1007/s11738-015-1924-6pt_BR
dc.languageen_USpt_BR
dc.publisherSpringerpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceActa Physiologiae Plantarumpt_BR
dc.subjectSomatic embryogenesispt_BR
dc.subjectCell suspensionpt_BR
dc.subjectBiotechnologypt_BR
dc.subjectSERKpt_BR
dc.subjectBBMpt_BR
dc.subjectSomatic Embryogenesis Receptor-like Kinase (SERK)pt_BR
dc.subjectBaby Boom (BBM)pt_BR
dc.titleGene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stagespt_BR
dc.typeArtigopt_BR

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