Utilização de resíduos agroindustriais como substratos para produção de enzimas pectinolíticas pelo agente biológico 'G088'

Abstract

Recently a large number of microorganisms isolated from different kind of materials have been selected in function of their ability to degrade the polysaccharides present in vegetal biomasses producing pectinases (pectinolitic enzymes). Several agro-industrial residues and sub-products like as orange bagasse, sugar cane bagasse, bread wheat, coffee spent-ground and other residues resultants of food processing are interesting substrates to production of pectinolitic enzymes. The application of residues is an alternative choice to obtain substrates solution questions about environmental pollution and to reduces the costs of production of enzymes. The pectinolitic enzymes, that degrade the pectin present in abundance on middle lamella and primary cell wall have a considerable commercial importance to several industrial applications such as improve the revenue of juice and clarification in the food process in beer industries, pharmaceutically and textile industries. The aim of this research was to evaluate the potential capacity of production of pectinase by fungi in different vegetal residues. In first step the fungi was inoculated in different substrates: orange bagasse, sugar cane bagasse, grape bagasse, passion fruit bagasse and coffee and rice spent-ground. The enzymatic activity of poligalacturonase (PG) and pectin metil esterase (PME) in substrates was evaluated and the better result from each enzyme was in function of time of cultivation and kind of substrate. There was done analysis of substrate composition and pH, humidity, ATT and pectin contents. The fungi produced pectinase, poligalacturonase (PG) and pectin metal esterase (PME) in all researched substrates.