Indução e composição bioquímica de calos de explantes obtidos de folha, caule e raiz de batata-doce

Abstract

The objective was to study the behavior of white-fleshed sweet potato leaf, stem and root explants in culture media with auxin and cytokinin and to biochemically analyze ex vitro plants and callus induced in culture media with increasing concentrations of sucrose, from explants of sweet potato with purple flesh and orange flesh. For this, two different experiments were carried out, where the first one studied the behavior of the explants of leaf, stem and root of the sweet potato Brazlândia Branca in culture medium containing 1 mg L ־ˡ of ANA (naphthalenoacetic acid) and 10 mg L ־ˡ of BAP (6-Benzylaminopurine), another supplemented with 3 mg L ־ˡ of 2.4D (dichlorophenoxyacetic acid), the third with 2 mg L ־ˡ of ANA plus 2 mg L ־ˡ of KIN (kinetin) and the control treatment without any kind of growth regulator. Assessments were made at 45 and 90 days. For all explants inoculated in culture medium with growth regulator, calluses were formed, leaf and stem explants showed greater mass than root explants and the culture medium that provided better callus development varied greatly depending on the inoculated explant. The second experiment studied the biochemical composition of the callus samples from explants obtained from the leaf, stem and root of purple-fleshed sweet potato, genotype 2018-72-1407, and orange, Beauregard cultivar, in culture medium supplemented with 30 mg L - ˡ, 60 mg L ־ˡ and 120 mg L ־ˡ of sucrose. The results showed that higher levels of sucrose in the culture medium can enhance the production of bioactive compounds in any of the inoculated explants.