Otimização da propagação in vitro e aclimatização de Paliavana sericiflora Benth

Abstract

Rock fields stand out as singular geosystems, boasting rich plant biodiversity with remarkable morphological, anatomical, physiological, and reproductive adaptations. These adaptations confer resilience to these species in the face of the harsh environmental conditions that characterize these environments, such as low organic matter content, shallow soils, and accentuated water deficit. Among the rupestrian campestrian flora, Paliavana sericiflora stands out, a species threatened with extinction according to the Red List of the Flora of Brazil. This plant not only has high ornamental potential but also proves to be attractive to local fauna, and can even be used in ecological restoration projects for these fragile ecosystems. The establishment of efficient protocols for micropropagation and acclimatization of P. sericiflora plays a crucial role in the species conservation strategy. This technique enables the mass production of seedlings with superior quality compared to other traditional seed propagation methods, significantly contributing to the preservation of this species. Micropropagation of P. sericiflora offers several advantages over conventional cultivation, such as the rapid production of uniform and disease-free seedlings. However, optimizing the components and in vitro cultivation conditions is crucial to maximize results and favor seedling acclimatization. In this sense, this study investigated the effect of carbohydrates, gas exchange systems, and light spectrum on the micropropagation of P. sericiflora. Acclimatization, a critical stage in micropropagation, represents a major challenge due to the abrupt environmental changes that can lead to significant seedling loss. To overcome this challenge, the study also proposes the use of a microcontainer system enriched with zinc nanoparticles (ZnO-NPs-NPs) to assist in the production and acclimatization of micropropagated P. sericiflora seedlings, promoting greater survival and growth.