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|Título :||Pectinases from a new strain of Cladosporium cladosporioides (Fres.) De Vries isolated from coffee bean|
|Autor:||Bastos, Sabrina Carvalho|
Pimenta, Carlos José
Dias, Disney Ribeiro
Chalfoun, Sara Maria
Angélico, Caroline Lima
Tavares, Lucas Silva
|Publicador:||World Science Research Journals (WSRJ)|
|Data da publicação:||2013|
|Referência:||BASTOS, S. C. et al. Pectinases from a new strain of Cladosporium cladosporioides (Fres.) De Vries isolated from coffee bean. World Journal of Agricultural Sciences, [S.l.], v. 9, n. 2, p. 167-172, 2013.|
|Abstract:||To examine tThe optimal conditions of cultivation for Cladosporium cladosporioides to produce and partially purify pectin methylesterase (PME) and polygalacturonase (PG) were examined under laboratory conditions . Activities of PME and PG were evaluated after 10, 15 and 20 days of incubation at 28°C in rice solid medium. Enzyme extraction was carried out by homogenization in buffer solutions at different pH (4, 5 and 6) followed by precipitation with ammonium sulfate [(NH4)2SO4] at 20, 40 and 60% saturation. After 10 days of incubation, the highest activities for PG (105.5 U/mL) and PME (1480 U/mL) in the crude extract was observed. Benzoate buffer (pH 4.0) was the best buffer for enzymes extraction. The final yield of 108.7% for PME and 10.6% for PG and a purification index of 14.2 and 1.4 for PME and PG, respectively, was obtained after saturation with 60%. The ideal conditions for obtained and pre-purification of PME and PG were 10 days of incubation, extraction with benzoate buffer pH 4.0 and precipitation with 60% of (NH4)2SO4. This is the first report on of pectinases pectinolytic enzymes produced by from C. cladosporioides and provides information about growth conditions for production and pre-purification of PME and PG.|
|Aparece nas coleções:||DCA - Artigos publicados em periódicos|
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