Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/4487
Título : Pectinases from a new strain of Cladosporium cladosporioides (Fres.) De Vries isolated from coffee bean
Autor: Bastos, Sabrina Carvalho
Pimenta, Carlos José
Dias, Disney Ribeiro
Chalfoun, Sara Maria
Angélico, Caroline Lima
Tavares, Lucas Silva
Palavras-chave: Pectinolytic enzymes
Filamentous fungi
Pectin
Coffee fermentation
Publicador: World Science Research Journals (WSRJ)
Data da publicação: 2013
Referência: BASTOS, S. C. et al. Pectinases from a new strain of Cladosporium cladosporioides (Fres.) De Vries isolated from coffee bean. World Journal of Agricultural Sciences, [S.l.], v. 9, n. 2, p. 167-172, 2013.
Abstract: To examine tThe optimal conditions of cultivation for Cladosporium cladosporioides to produce and partially purify pectin methylesterase (PME) and polygalacturonase (PG) were examined under laboratory conditions . Activities of PME and PG were evaluated after 10, 15 and 20 days of incubation at 28°C in rice solid medium. Enzyme extraction was carried out by homogenization in buffer solutions at different pH (4, 5 and 6) followed by precipitation with ammonium sulfate [(NH4)2SO4] at 20, 40 and 60% saturation. After 10 days of incubation, the highest activities for PG (105.5 U/mL) and PME (1480 U/mL) in the crude extract was observed. Benzoate buffer (pH 4.0) was the best buffer for enzymes extraction. The final yield of 108.7% for PME and 10.6% for PG and a purification index of 14.2 and 1.4 for PME and PG, respectively, was obtained after saturation with 60%. The ideal conditions for obtained and pre-purification of PME and PG were 10 days of incubation, extraction with benzoate buffer pH 4.0 and precipitation with 60% of (NH4)2SO4. This is the first report on of pectinases pectinolytic enzymes produced by from C. cladosporioides and provides information about growth conditions for production and pre-purification of PME and PG.
URI: http://www.idosi.org/wjas/wjas9(2)13/9.pdf
http://repositorio.ufla.br/jspui/handle/1/4487
Idioma: en
Aparece nas coleções:DCA - Artigos publicados em periódicos
DNU - Artigos publicados em periódicos

Arquivos associados a este item:
Não existem arquivos associados a este item.


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.