Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/10702
Título: Identificação do sexo e variabilidade genética em uma população de Astronotus ocellatus (Agassiz, 1831) por marcadores ISSR
Autores: Murgas, Luis David Solis
Paula, Daniella Aparecida de Jesus
Carvalho, Dulcinéia de
Carvalho, Josina Aparecida de
Palavras-chave: Marcadores moleculares
Sexagem
Peixe
Diversidade
Molecular markers
Sexing
Fish
Diversity
Data do documento: 17-Dez-2015
Editor: Universidade Federal de Lavras
Citação: PAIVA, I. M. Identificação do sexo e variabilidade genética em uma população de Astronotus ocellatus (Agassiz, 1831) por marcadores ISSR. 2015. 55 p. Dissertação (Mestrado em Ciências Veterinárias)-Universidade Federal de Lavras, Lavras, 2015.
Resumo: Studies conducted in order to investigate alternative methods for sexing fish species, such as Astronotus ocellatus, using reduced sexual dimorphism, are extremely important to facilitate reproduction management techniques. The objective of this study was to determine the feasibility of manual sexing, widely used by fish farmers and hobbyists, and the identification of specific molecular markers for a particular sex using ISSR markers. The latter technique also generates genetic diversity and similarity data, and is important for conservation studies. Manual sexing was performed by macroscopic analysis of the urogenital papilla. Fin and gonad samples of 30 A. ocellatus (±83,32g e ±15, 96 cm) were collected for DNA extraction and histology, respectively. For DNA extraction, we adopted the NaCl protocol (SAMBROOK, 1989). Quality samples were used for amplification, using universal ISSR primers with subsequent separation of the generated fragments by electrophoresis, and assessment of similarity and genetic diversity levels. The manual sexing did not appear as a viable technique to distinguish the sexes for this species, given the occurrence of 37% of errors during selection. Likewise, the band profiles generated by the amplification with ISSR primers could not distinguish a specific pattern for one sex. The percentage of polymorphism (64,4%) obtained, as well as He and I indexes (0.225 ± 0.030 and 0.375 ± 0.044, respectively) suggest moderate diversity, indicating that marked captive populations do not always decrease in genetic diversity.
URI: http://repositorio.ufla.br/jspui/handle/1/10702
Aparece nas coleções:Ciências Veterinárias - Mestrado (Dissertações)



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