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metadata.artigo.dc.title: Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit
metadata.artigo.dc.creator: Carvalho, Milene Alves de Figueiredo
Paiva, Renato
Stein, Vanessa Cristina
Herrera, Raírys Cravo
Porto, Jorge Marcelo Padovani
Vargas, Daiane Peixoto
Alves, Eduardo
metadata.artigo.dc.subject: Passifloraceae
Optic microscope
Tissue culture
metadata.artigo.dc.publisher: Instituto de Tecnologia do Paraná - Tecpar 2014
metadata.artigo.dc.identifier.citation: CARVALHO, M. A. de F. et al. Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit. Brazilian Archives of Biology and Technology, Curitiba, v. 57, n. 6, p. 851-859, Nov. /Dec. 2014.
metadata.artigo.dc.description.abstract: This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.
metadata.artigo.dc.language: en_US
Appears in Collections:DFP - Artigos publicados em periódicos

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