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metadata.artigo.dc.title: Large-scale, high-efficiency production of coffee somatic embryos
metadata.artigo.dc.creator: Maciel, Anna Lygia de Rezende
Rodrigues, Filipe Almendagna
Pasqual, Moacir
Carvalho, Carlos Henrique Siqueira de
metadata.artigo.dc.subject: Vegetative propagation
Tissue culture
Cotyledonary embryos
Propagação vegetativa
Cultura de tecidos
Embriões cotiledonares
metadata.artigo.dc.publisher: Crop Breeding and Applied Biotechnology Apr-2016
metadata.artigo.dc.identifier.citation: MACIEL, A. L. de R. et al. Large-scale, high-efficiency production of coffee somatic embryos. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 16, n. 2, p. 102-107, Apr./June 2017.
metadata.artigo.dc.description.abstract: The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.
metadata.artigo.dc.language: en_US
Appears in Collections:DAG - Artigos publicados em periódicos

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