Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/40086
Title: Protein recovery from barbados gooseberry (Pereskia aculeata Miller) leaves by salting out and isoelectric precipitation
Keywords: Barbados gooseberry - Mucilage
Protein concentrate
Salting out extraction
Ora-pro-nobis - Mucilagem
Concentrado proteico
Issue Date: 2019
Publisher: Academia Mexicana de Investigación y Docencia en Ingeniería Química
Citation: MORAIS, L. C. et al. Protein recovery from barbados gooseberry (Pereskia aculeata Miller) leaves by salting out and isoelectric precipitation. Revista Mexicana de Ingeniería Química, Ciudad de México, v. 18, n. 2, p. 419-430, 2019.
Abstract: This study evaluated the protein recovery from extract of Barbados gooseberry leaves by the synergistic effect of salting out and isoelectric precipitation using a factorial design, varying salt type ((NH4)2SO4, NaCl and KCl), salt concentration and temperature. Temperature elevation increased salting out effect. The optimum salt concentration was 0.5 mol/L for (NH4)2SO4 (66%), 1.5 mol/L for KCl (58%), and 2.5 for NaCl (62%). Research on isoelectric precipitation were performed. The most effective condition was found at 85 °C, using (NH4)2SO4 at 0.8 mol/L to 1.0 mol/L and pH = 1.0. Results showed the effectiveness of these effects on protein precipitation.
URI: http://repositorio.ufla.br/jspui/handle/1/40086
Appears in Collections:DCA - Artigos publicados em periódicos



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