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http://repositorio.ufla.br/jspui/handle/1/40086| Title: | Protein recovery from barbados gooseberry (Pereskia aculeata Miller) leaves by salting out and isoelectric precipitation |
| Keywords: | Barbados gooseberry - Mucilage Protein concentrate Salting out extraction Ora-pro-nobis - Mucilagem Concentrado proteico |
| Issue Date: | 2019 |
| Publisher: | Academia Mexicana de Investigación y Docencia en Ingeniería Química |
| Citation: | MORAIS, L. C. et al. Protein recovery from barbados gooseberry (Pereskia aculeata Miller) leaves by salting out and isoelectric precipitation. Revista Mexicana de Ingeniería Química, Ciudad de México, v. 18, n. 2, p. 419-430, 2019. |
| Abstract: | This study evaluated the protein recovery from extract of Barbados gooseberry leaves by the synergistic effect of salting out and isoelectric precipitation using a factorial design, varying salt type ((NH4)2SO4, NaCl and KCl), salt concentration and temperature. Temperature elevation increased salting out effect. The optimum salt concentration was 0.5 mol/L for (NH4)2SO4 (66%), 1.5 mol/L for KCl (58%), and 2.5 for NaCl (62%). Research on isoelectric precipitation were performed. The most effective condition was found at 85 °C, using (NH4)2SO4 at 0.8 mol/L to 1.0 mol/L and pH = 1.0. Results showed the effectiveness of these effects on protein precipitation. |
| URI: | http://repositorio.ufla.br/jspui/handle/1/40086 |
| Appears in Collections: | DCA - Artigos publicados em periódicos |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ARTIGO_Protein recovery from barbados gooseberry (Pereskia aculeata Miller) leaves by salting out and isoelectric precipitation.pdf | 1,14 MB | Adobe PDF | View/Open |
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