Efeito da bebida de café (Coffea arábica L.) sobre o estresse oxidativo e agregação plaquetária em ratos diabéticos

Abstract

This study aimed at verifying the influence of the coffee beverage in different biological systems of alloxan-induced diabetic Wistar rats. In all experiments, the Coffea arabica beverage was used, boiled and filtered, prepared at the moment of use. The composition of total chlorogenic acids, total phenolic contents, caffeine, trigonelina and coffee niacin were determined. The animals were divided into non-diabetic and diabetic (alloxan-induced via intraperitoneal) groups, animals treated by gavage with water, with the aminoguanidine and apocynin inhibitors, and with the coffee beverage for a period of 50 days, in standardized doses and concentrations. The neutrophils’ recruitment was performed using sodium caseinate via intraperitoneal, the serum and total blood biological samples were obtained by cardiac puncture and the citrated plasma by abdominal aorta puncture. The tests were performed to evaluate the glycemic profile through the doses of fasting blood glucose and HbA1c, the renal profile through doses of urea and creatinine, circulating levels of serum fluorescent advanced glycation end products – AGEs and complete blood count. The generation of oxygen reactive species - ORS pool was evaluated by luminol amplified chemiluminescence and activity of the NOX2 enzyme, through the generation of superoxide anion by means of the cytochrome C reduction trial, using the PMA as activator, the phagocytic and microbicide activities of peritoneal neutrophils in contrast to Candida albicans. Platelet aggregation was evaluated in contrast to the ADP agonist. With the analyses of the obtained data, it was noticed that the ingestion of the coffee beverage did not affect the glycemic profile of the animals, however, the formation of AGEs was reduced as well as the platelet aggregation in the adopted experimental model. The aminoguanidine was efficient in inhibiting the formation of AGEs and, in addition, inhibited platelet aggregation. In addition, the ingestion of coffee beverage did not interfere in the renal function, reduced the generation of ORS of the animals and the generation of superoxide anion in diabetic the animals, did not interfere with the phagocyte and microbicide activities of peritoneal neutrophils of the studied animals in contrast to Candida albicans.