Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/42983
Title: Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatography
Other Titles: Padronização de um método analítico para quantificação de ocratoxina A em grãos de café verde por cromatografia líquida de alta eficiência
Cuantificación de ocratoxina A en granos verdes de café mediante cromatografía líquida de alta eficiencia
Keywords: Cromatografia líquida de alta eficiência
Fungo
Toxina
Segurança alimentar
Café - Qualidade
High performance liquid chromatography
Fungus
Toxin
Food safety
Coffee - Quality
Issue Date: Jun-2020
Publisher: CDRR Editors
Citation: SANTIAGO, W. D. et al. Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatography. Research, Society and Development, Vargem Grande Paulista, v. 9, n. 8, p. e39985070, 2020. DOI: 10.33448/rsd-v9i8.5070.
Abstract: Nowadays, Brazil is the largest producer and exporter of coffee, also the second largest consumer of the beverage. The importance of ensuring food safety for consumers has influenced research to improve and monitor the final product quality. Coffee is a product that presents a high risk of fungal contamination, which can result in the presence of mycotoxins and poses risks to human and animal health. Therefore, this study aimed to standardize a chromatographic method to test and quantify ochratoxin A in 13 samples of green coffee beans. The green coffee beans were stored in sheds without temperature or humidity control. Samples were ground, and the analyte was extracted by a 3% methanol:sodium bicarbonate (1:2 v/v) solution. Ochratoxin A was quantified in a high performance liquid chromatograph. The method was validated by testing the selectivity, linearity, accuracy, limits of detection and quantification. The method presented robustness to the tested parameters and among the analyzed samples. Ochratoxin A was detected above the limit established by the legislation (75.19 µg kg-1) only in one sample. Overall, the storage of green coffee beans in these sheds was adequate, since 12 samples had a low content of ochratoxin A and they were within the limit established by legislation. Therefore, food safety was guaranteed without any severe mycotoxin contamination.
URI: http://repositorio.ufla.br/jspui/handle/1/42983
Appears in Collections:DFP - Artigos publicados em periódicos
DQI - Artigos publicados em periódicos



This item is licensed under a Creative Commons License Creative Commons