Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/50423
Title: Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys
Keywords: In vitro culture
Plant cloning
Plant growth regulator
Rooting container
Vegetative propagation
Cultura in vitro
Plantas - Clonagem
Reguladores de crescimento vegetal
Propagação vegetativa
Issue Date: Apr-2022
Publisher: Scion
Citation: FARIA, J. C. T. et al. Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys. New Zealand Journal of Forestry Science, [S.I.], v. 52, n. 10, 2022. DOI: https://doi.org/10.33494/nzjfs522022x139x.
Abstract: Background: Micropropagation, an in vitro vegetative propagation technique using small propagules is one of the main applications of plant tissue culture. It can be used to clone specific plants with desired traits and reduce the cost of plant propagation. In this study, we developed a protocol for micropropagation of Eucalyptus microcorys F.Muell using a selected mature tree, in which we tested various combinations of different culture media and evaluated the use of biodegradable polyester-based microvessels during the adventitious rooting and acclimatisation phases. Methods: Epicormic shoots were used as an explant source. After the in vitro explant establishment and multiplication, we tested 8 combinations of BAP, NAA and IBA in the elongation phase. Three types of microvessels were tested in the adventitious rooting phase and acclimatisation of the microcuttings. Results: Epicormic shoots had an establishment percentage of 40.6% and a total of 820 explants were generated by the 11th subculture, with an average of 12 buds per explant. Best shoot elongation results were achieved with BAP (0.05 mg L-1) + NAA (1 mg L-1) and BAP (0.05 mg L-1) + NAA (1 mg L-1) + IBA (1 mg L-1) combinations, whereas microvessel types M2 and M3 provided higher rooting and acclimatisation. According to the results of ISSR markers, at the end of 535 days of in vitro cultivation, cloning was successful between acclimatised micro-plantlets and the parent plant. Conclusions: The micropropagation protocol using microvessels was efficient in producing E. microcorys clonal micro-plantlets and is recommended for further studies with this species, and for testing in the micropropagation of other species.
URI: http://repositorio.ufla.br/jspui/handle/1/50423
Appears in Collections:DAG - Artigos publicados em periódicos
DCF - Artigos publicados em periódicos
DEG - Artigos publicados em periódicos



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