Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/50826
Title: Application of Congo red dye as a molecular probe to investigate the kinetics and thermodynamics of the formation processes of arachin and conarachin nanocomplexes
Keywords: Plant-based protein
Nanocarriers
Transition complex
Surface plasmon resonance
Hydrophobic interactions
Hydrophilic interactions
Congo red (CR)
Peanut proteins
Issue Date: 1-Aug-2022
Publisher: Elsevier
Citation: REZENDE, J. de P. et al. Application of Congo red dye as a molecular probe to investigate the kinetics and thermodynamics of the formation processes of arachin and conarachin nanocomplexes. Food Chemistry, [S.l.], v. 384, p. 1-10, Aug. 2022. DOI: 10.1016/j.foodchem.2022.132485.
Abstract: The thermodynamics and kinetics of arachin-Congo red (ARA-CR) and conarachin-Congo red (CON-CR) interactions were studied using surface plasmon resonance. KCl led to a reduction of up to 55% in the values of the associated kinetic constants, but it had less influence on the dissociation rates (less than 12%). The change in ionic strength had little effect on the thermodynamic stability of the complexes, but it did reduce their affinities ( Kb(25ºC)[ARA-CR]º from 3.52 to 2.44 × 103 M−1 and ( Kb(25ºC)[CON-CR]º from 15.1 to 12.5 × 103 M−1). The shielding of the electrical double layer favored ARA-CR hydrophilic interactions (ΔHº [ARA-CR]º decreased from −30.60 to −42.98 kJ mol−1). On the other hand, hydrophobic interactions came to dominate during the formation of [CON-CR]º (ΔHº [CON-CR]º( increased from −11.21 to 28.34 kJ mol−1 and TΔSº 25º[CON-CR]º increased from 12.64 to 51.73 kJ.mol−1). The data presented here improve our understanding of plant-based protein nanocarriers of small bioactive molecules.
URI: https://www.sciencedirect.com/science/article/pii/S0308814622004472
http://repositorio.ufla.br/jspui/handle/1/50826
Appears in Collections:DCA - Artigos publicados em periódicos

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