Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/53309
Title: Adsorption and immobilization of b-glucosidase fromThermoascus aurantiacus on macroporous cryogel by hydrophobic interaction
Keywords: Adsorption
β-glucosidase
Cryogel
Hydrophobic interaction
L-phenylalanine
Issue Date: Jun-2022
Publisher: Taylor & Francis
Citation: MÓL, P. C. G. et al. Adsorption and immobilization of b-glucosidase fromThermoascus aurantiacus on macroporous cryogel by hydrophobic interaction. Preparative Biochemistry & Biotechnology, [S.l.], June 2022. DOI: 10.1080/10826068.2022.2081860.
Abstract: Enzyme immobilization has been reported as a promising approach to improving parameters such as thermal stability, pH and reusability. In this study, a polyacrylamide cryogel functionalized with L-phenylalanine was prepared to be used in the adsorption of β-glucosidase from Thermoascus aurantiacus, aiming at its separation and also its immobilization on the cryogel matrix. The enzyme was produced by solid state fermentation. First, the adsorption was studied as a function of the pH and the resulting yield (Y, %) and purification factor (PF, dimensionless) were determined (1.57–5.13 and 64.19–91.20, respectively). The PF and yield from eluate samples obtained at pH 3.0 were the highest (5.13 and 91.20, respectively). Then, β-glucosidase was immobilized on the hydrophobic cryogel and the recovery activities (%) were determined as a function of temperature and in the presence of different saline solutions. The values ranged from 14.45 to 45.97. As expected, salt type and ionic strength affected the activity remained in the immobilized β-glucosidase. The average bioreactor activity was 39.9 U/g of dry cryogel and its operational stability was measured, with no decrease in activity being observed during seven cycles. Kinetic parameters of free and immobilized enzyme were determined according to different models.
URI: https://www.tandfonline.com/doi/abs/10.1080/10826068.2022.2081860
http://repositorio.ufla.br/jspui/handle/1/53309
Appears in Collections:DCA - Artigos publicados em periódicos

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.