Germinação de sementes e propagação in vitro de sucupira branca [Pterodon pubescens (Benth.) Benth]

Abstract

Pterodon pubescens (Benth.) Benth. is a essence native to Brazilian cerrados, reaching up to 16 meters in height. The oil of fruit is very enjoyed in folk medicine in sore and meumatic infections, it protects from cercaria infections. The wood posses high natural resistence to rotting, being regarded as one ofthe most resistent woods for railroad sleepers. The objectives ofthe present work were to identify the best "in vitro" and "ex vitro" germination conditions for the seed, embryo and embryonic axes ofthe seedling's development, in addition to determinating a methodology for in vitro multiplication. Experiments were conducted aiming at the achievement ofin vitro germination by means ofthe use ofmodifíed MS basic médium, in liquid médium, different gelifyings, caps and light conditions the establishment of nodal segments were studied by employing the MS and WPM basic médium. In the muki-sprouting experiment, four different combined concentrations ofANA and BAP were tested. lhe best type of cap for embryo germination was the one of aluminum paper mould together with liquid médium the nodal segments were best established in WPM médium. Up to 75 shoots/segment were obtained, with the nodal segment, inoculated at the horizontal posftion in the MS cumire médium with halfthe concentration ofsalts, supplemented with 0,5 uM BAP.