Phosphorous and sulfur nutrition modulate antioxidant defenses in Myracrodruom urundeuva plants exposed to arsenic

Abstract

We investigated if plant nutrition and antioxidant system activation are correlated features of arsenic (As)-tolerance in Myracrodruom urundeuva. Plants were grown for 120 days in substrates with 0, 10, 50 and 100 mg As kg−1 and its As-tolerance was demonstrated. As-concentrations greater than 10 mg kg−1 decreased plant growth and photosynthesis but did not induce plant death. Plants coupled alterations in stomatal conductance and transpiration to avoid As-deleterious effects to the photosynthetic apparatus. As-toxicity in M. urundeuva was due to lipid peroxidation induced by hydrogen peroxide accumulation. Ascorbate peroxidase (APX) and gluthatione peroxidase (GPX) had central roles in hydrogen peroxide (H2O2) scavenging in leaves, and their activities were linked to changes in redox potentials (ascorbate and glutathione pools). APX and GPX inactivation/degeneration led to H2O2 accumulation and related lipid peroxidation. Increased phosphorus (P) and sulfur (S) concentrations in leaves were related to increased APX and GPX activities by stimulating increases in glutathione biosynthesis. We concluded that P and S nutrition were directly linked to As-tolerance in M. urundeuva plants by increasing antioxidant system activities.