Produção de enzimas pectinolíticas e seletividade de produtos fitossanitários sobre o agente biológico G088

Abstract

For obtaining microbial enzymes, the selection of a microorganism is necessary. The specific strains of microorganisms employed in the production of enzymes are originally obtained by isolation from natural sources or from the cultures maintained in established collections. Recently, a great number of microorganisms, isolated from different materials have been selected by their ability of degrading the polysaccharides present in the plant biomass producing pectinases (pectinolytic enzymes). The pectonolytic enzymes, which degrade the pectin present in the media lamella and primary cell wall, have a great commercial importance for a number of applications industrial applications such as to improve juice yields and clarification in the food industry, brewery and pharmaceutical and textile industry. The selectivity of agrotoxic chemicals is a process in which organisms such as fungi develop tolerance to those complexes. Being aware of the great prominence of the use of pectinases in the industrial sector, it was aimed in this work to evaluate several isolates of biologic agent through the determination of velocity characteristics and growth rate in culture medium and production of pectinolytic enzymes; compare the isolate superior as regards the set of previous characteristics with the main commercial producers through the production curves, pectinmethylesterase (PME) and ploygalacturonase (PG), validating in this way their industrial potential. In addition, the selectivity of the products (defensive) usually utilized in coffee production aiming the preservation of bioprotector biologic agent of coffee quality. Isolates GO76, GO77, GO79, GO80, GO81, GO82, GO84, GO87 and GO88 which showed superior IVCM. Isolate GO88 which presented average activity superior to the other for production of PME and PG, its being also superior to Aspergillus niger and Rhizopus sp. The chemicals Biopirol (T 10) and Envidor (T 14) were more selective concerning the mycelial velocity index and % of growth inhibition of the biologic agent GO88.