Diversidade e eficiência de bactérias isoladas de nódulos de diferentes leguminosas da região do Alto Solimões, AM

Abstract

This work aimed to isolate and characterize phenotypically and genotypically bacterial isolates from nodules collected from 16 leguminous species: Acacia multipinnata, Calopogonium mucunoides, Desmodium adscendens, Dalbergia inundata, Entada sp, Inga caynnensis, Inga edulis, Inga heterophylla, Inga umbellifera, Inga venusta, Machaerium floribundum, Mimosa pudica, Mimosa quadrivalvis, Pueraria phaseoloides, Piptadenia suaveolens and Pitryocarpa pteroclada. For this, collections were made in several sampling points allocated in 5 different land use systems (LUSs) in Alto Solimões, AM region: young secondary forest, old secondary forest, agroforest, agriculture and pasture. Three nodules of each species in each LUS were selected for isolation, from which all morphotypes obtained in plates were isolated and characterized culturally. Besides that the nitrogen fixing efficiency in symbiosis with Macroptilium atropurpureum were evaluated for representative isolates of cultural groups. Isolates which did not nodulate siratro and stimulate plant growth statistically higher than the absolute control were assessed regarding the production of ndole-3-acetic acid. 16S rDNA gene were partially sequenced for representative isolates and submitted to phylogenetic analysis.