Direct organogenesis and Leaf-Anatomy modifications in vitro of neoregelia concentrica (Vellozo) L.B. Smith (bromeliaceae)

Abstract

Tissue culture can contribute in the multiplication of several species with commercial interest, like the bromeliads. It was aimed to evaluate cytokinins and its concentrations in the multiplication and leaf structure of Neoregelia concentrica (Vellozo) L.B. Smith. Previously In vitro-established N. concentrica plants were inoculated in MS medium supplemented with 6-benzylaminopurine (BAP) or Kinetin (KIN) with concentrations 0.0, 5.0, 10.0 and 15.0 μM. For the anatomic analyses tree plants of each treatment were randomly sampled at 60-day growth. Significant differences were verified in the evaluated characteristics due to the treatments. The raise in cytokinin concentrations induced a higher percentage and average number of explants with shoots. BAP provided higher averages when compared to KIN. The cytokinin use modified the epidermal structure and induced a larger thickening of the water-storage and chlorophyll parenchymas. The use of 15.0 μM BAP was efficient in the In vitro multiplication and in the leaf tissue development of N. concentrica.