Enzymatic and microsatellite markers for the characterization of millet cultivars

Abstract

In this study the objective was to characterize millet cultivars through enzymes and microsatellite markers and select microsatellite primers and enzymatic systems to identify cultivars. Twelve millet cultivars, which were tested in seven enzymatic systems (ADH, CAT, EST, GOT, MDH, IDH and ACP) and 123 pairs of microsatellite primers were evaluated. By the enzymes ACP and CAT was not observed sufficient polymorphism to cultivars differentiation. Sixty pairs of microsatellite primers were selected. About the enzymatic systems evaluated, EST presented more polymorphic to distinguish cultivars. By the primers PSMP2008, PSMP2045 and PSMP2056 is possible to distinguish all cultivars used in this study. It is possible to identify millet cultivars by means of enzymes and microsatellite markers.