Expressão gênica diferencial por CDNA-RAPD entre cultivares de feijão inoculadas por Sclerotinia sclerotiorum

Abstract

The knowledge of the transcriptional profile of genotypes under specific treatments has been considered as a tool of great relevance for the molecular understanding of the patossystems, aiming the knowledge of the resistance. Beans are one of the most important crops of the Brazilian economy and susceptible to pathogens. White mold, a fungal disease caused by the pathogen Sclerotinia sclerotiorum, has the ability to drastically reduce crop yields, under favorable conditions these losses may be irreversible. Therefore, this work tried to understand directly and indirectly the action of the pathogen against the bean culture. Therefore, this study aimed to: (i) evaluate adjustments of different protocols of commercial Total RNA extraction kits using bean stems inoculated with the pathogen Sclerotinia sclerotiorum and, (ii) verify, by the cDNA-RAPD technique, the existence of variability of expression, and to obtain information about the transcriptome of susceptible and white mold resistant cultivars when inoculated by the pathogen. The use of the RAPD technique in express material using 57 decamer primers allowed the visualization of amplification of 255 loci, with about 136 of these polymorphic, thus proving the existence of variability of genetic expression, with the identification of putative loci of interest , through the qualitative and quantitative differences observed between the inoculated and uninoculated treatments of both strains.