dissertação
Desenvolvimento de metodologia eletroanalítica de tricaína em peixes (Oreochromis niloticus)
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UNIVERSIDADE FEDERAL DE LAVRAS
Faculdade, Instituto ou Escola
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Programa de Pós-Graduação
DQI - Departamento de Química
Agência de fomento
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Resumo
Durante o processo de produção, alguns alimentos podem ser expostos a agentes químicos contaminantes, representando um risco à saúde humana. Na aquicultura há uma grande diversidade de drogas, dentre elas os anestésicos, que são utilizados para insensibilizar os peixes e reduzir o estresse animal. Porém, o processo de anestesia pode proporcionar traços residuais, contaminando o pescado. Dessa forma, é necessário o desenvolvimento de protocolos analíticos que possibilitem a detecção rápida, prática e precisa desses anestésicos no filé do pescado. As técnicas eletroquímicas representam uma importante fonte de pesquisa, uma vez que oferecem baixo custo, com sensibilidade e seletividade, além de apresentarem a possibilidade de serem transportadas para sistemas portáteis. Sendo assim, o presente trabalho foi realizado com o objetivo de desenvolver uma metodologia para a determinação de tricaína em filés de peixes, por meio das técnicas eletroquímicas. Foram utilizadas as modalidades cíclica, varredura linear, pulso diferencial e onda quadrada para o desenvolvimento da metodologia. A tricaína apresentou um comportamento de oxidação irreversível, com dois picos anódicos em potencial de 0,8 V (pico I) e 1,3 V (pico II). O processo de transferência de massa foi controlado por difusão com ocorrência de reações químicas sem complicações cinéticas. A melhor relação da intensidade de corrente de pico anódico e largura de pico à meia altura foi obtida com a modalidade pulso diferencial. O método eletroquímico desenvolvido apresentou limites de detecção de 2,7 µmol L-1 e de quantificação de 8,2 µmol L-1. Foram realizados dois métodos de extração da tricaína no filé de peixe e o método I apresentou maior eficiência, com recuperação de 106,13%.
During the production process, some foods can be exposed to chemical agents contaminants, presenting a risk to human health. In aquaculture there is a great variety of drugs, among them, anesthetics which are used to desensitize the fish and reduce animal stress. However the anesthesia process can provide residual traces, contaminating the fish. Thus, it is necessary to develop protocols that enable the rapid, practical and accurate detection of these anesthetics in the fish fillet. Electrochemical techniques represent an important source of research, as they offer low-cost, sensitivity and selectivity, in addition having the possibility to be transported to portable systems. Thus, the goal of the present work was to develop a methodology for the tricaine determination in fish fillets by means of electrochemical techniques. For the methodology development were used the cyclic modes, linear sweep, differential pulse and square wave. The tricaine presented an irreversible oxidation behavior with two anodic peak in potential of 0.8 V (peak I) and 1.3 V (peak II). The mass transfer process was controlled by diffusion with occurring chemical reactions without complications. The best ratio of current intensity of the anodic peak and peak width at half height was obtained by differential pulse mode. The electrochemical method developed showed a detection limit of 2.7 µmol L-1 and quantification 8.2 µmol L-1. Two methods of tricaine extraction in fish fillet were made, the method I showed more efficient with recovery of 106.13%.
During the production process, some foods can be exposed to chemical agents contaminants, presenting a risk to human health. In aquaculture there is a great variety of drugs, among them, anesthetics which are used to desensitize the fish and reduce animal stress. However the anesthesia process can provide residual traces, contaminating the fish. Thus, it is necessary to develop protocols that enable the rapid, practical and accurate detection of these anesthetics in the fish fillet. Electrochemical techniques represent an important source of research, as they offer low-cost, sensitivity and selectivity, in addition having the possibility to be transported to portable systems. Thus, the goal of the present work was to develop a methodology for the tricaine determination in fish fillets by means of electrochemical techniques. For the methodology development were used the cyclic modes, linear sweep, differential pulse and square wave. The tricaine presented an irreversible oxidation behavior with two anodic peak in potential of 0.8 V (peak I) and 1.3 V (peak II). The mass transfer process was controlled by diffusion with occurring chemical reactions without complications. The best ratio of current intensity of the anodic peak and peak width at half height was obtained by differential pulse mode. The electrochemical method developed showed a detection limit of 2.7 µmol L-1 and quantification 8.2 µmol L-1. Two methods of tricaine extraction in fish fillet were made, the method I showed more efficient with recovery of 106.13%.
Abstract
During the production process, some foods can be exposed to chemical agents contaminants, presenting a risk to human health. In aquaculture there is a great variety of drugs, among them, anesthetics which are used to desensitize the fish and reduce animal stress. However the anesthesia process can provide residual traces, contaminating the fish. Thus, it is necessary to develop protocols that enable the rapid, practical and accurate detection of these anesthetics in the fish fillet. Electrochemical techniques represent an important source of research, as they offer low-cost, sensitivity and selectivity, in addition having the possibility to be transported to portable systems. Thus, the goal of the present work was to develop a methodology for the tricaine determination in fish fillets by means of electrochemical techniques. For the methodology development were used the cyclic modes, linear sweep, differential pulse and square wave. The tricaine presented an irreversible oxidation behavior with two anodic peak in potential of 0.8 V (peak I) and 1.3 V (peak II). The mass transfer process was controlled by diffusion with occurring chemical reactions without complications. The best ratio of current intensity of the anodic peak and peak width at half height was obtained by differential pulse mode. The electrochemical method developed showed a detection limit of 2.7 µmol L-1 and quantification 8.2 µmol L-1. Two methods of tricaine extraction in fish fillet were made, the method I showed more efficient with recovery of 106.13%.
Descrição
Dissertação apresentada à Universidade Federal de Lavras como parte das exigências do Programa de Pós-Graduação em Agroquímica, para a obtenção do título de Mestre.
Área de concentração
Agroquímica
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Procedência
Impacto da pesquisa
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DOI
Citação
LIMA, R. M. F. de. Desenvolvimento de metodologia eletroanalítica de tricaína em peixes (Oreochromis niloticus). 2014. 106 p. Dissertação (Mestrado em Agroquímica) - Universidade Federal de Lavras, Lavras, 2014.