Estabelecimento, multiplicação e alongamento in vitro de Eucalyptus benthamii Maiden & Cambage x Eucalyptus dunnii Maiden

Abstract

This work aimed to evaluate different concentrations of active chlorine (NaOCl) in explant asepsis for the establishment in vitro as well as of benzylaminopurine (BAP) and naphthalene acetic acid (NAA) in the multiplication and elongation of Eucalyptus benthamii x Eucalyptus dunnii. Ministumps, which supply shoots for introduction in vitro, were cultivated in a clonal mini garden under semi-hydroponic system. Nodal segments of clones H12, H19 and H20 were disinfested with 0.5, 1.0, 1.5 and 2.0% (v/v) of active chlorine during 10 minutes and inoculated in MS medium. In the multiplication phase, culture ½MS medium supplemented with 0, 0.25, 0.50, 0.75 and 1.0 mg L-1 of BAP was used. In the elongation phase, ½MS medium supplemented with 0, 0.25, 0.50, 0.75 and 1.0 mg L-1 of NAA was used. No interaction occurred between the studied levels, with 45%, 46% and 66% of establishment of clones H12, H19 and H20, respectively. The BAP concentration that resulted in the highest axillary’s bud proliferation for clone H12 at 60 days was estimated in the range of 0.25 to 0.30 mg L-1. At 60 days, the range between 0.25 to 0.75 mg L-1 of NAA, promoted the highest number of elongated shoots for H12 clone.