Morfologia de calos de Eucalyptus urophylla cultivados in vitro sob concentrações de boro e cálcio

Abstract

Boron and calcium are essential nutrients in plant development, but there are few registers of the common action of these nutrients in vegetable. In order to identify and quantify the possible implications of these micro and macronutrients in the morphogenetic development of Eucalyptusurophylla, callus were cultivated invitro with different concentration of boron (0, 25, 50, 100 e 200 μM H3BO3) and calcium (0, 3.75, 7.50, 11.25 e 15.00 mM CaCl2). The experiment was completely randomized design in factorial arrangement (5 x 5) with 5 replications. It was evaluated after 21th and 31th days of culture the relation of external and internal morphology. It could be verified that in two evaluations periods the combined ofboron and calcium had intervened in aspects of the internal e external morphology that had caused direct answers in visual aspects of the calluses. The combinations of 25 μM B with 0, 3.75, 7.50, 11.25 e 15.00 mM Ca in 21th day, were less harmful of the roots favoring appropriately organ development. The production of dry matter weight was directly influenced by boron concentration and this effect was constant for all calcium concentration and for both evaluated days