A new alternative use for coffee pulp from semi‐dry process to β‐glucosidase production by Bacillus subtilis

Abstract

Coffee is among the most preferred nonalcoholic drinks, and its consumption is distributed globally. During the coffee fruiting process, however, a large amount of waste is generated in the form of pulp, mucilage, husks, and water waste. The pulp and mucilage have the chemical composition to support the growth of micro‐organisms and the production of value‐added product. The aim was testify pulp coffee can be considered as carbon and inductor source for β‐glucosidase by Bacillus subtilis CCMA 0087. The response surface methodology (RSM) based on a central composite rotatable design (CCRD) was employed for this optimization. The methodology used in the optimization process was validated by testing the best conditions obtained and comparing them with the values predicted by the model. The highest β‐glucosidase production (22·59 UI ml−1) was reached in 24 h of culturing at coffee pulp concentration of 36·8 g l−1, temperature of 36·6°C, and pH of 3·64.