Caracterização de suspensões celulares e obtenção de sementes sintéticas de Coffea arabica cv. Catiguá

Abstract

We analyzed, in this work, the histological and molecular characteristics, and the regeneration capacity of embryogenic cell suspensions, as well as the optimization of a protocol for synthetic seeds production, from somatic embryos of C. arabica cv. Catiguá. According to the results, there was no differences, either in the histological aspects of the samples or in the relation of gene expression with the regenerative potential of ECS. However, we found a transient induction of relation between BBB and SERK, high levels of SERK expression in the various stages of embryos development, and basal levels of WUS expression for all samples of ECS, by being significantly expressed from the cordiforme/torpedo stage. It was possible identifying that lineages of ECS, known as embryogenics by their visual appearance, did not act the same way when analyzed their regeneration patterns. We also found that the embryos maturation on liquid MGM culture medium was favourable for somatic embryos obtaining in the torpedo and cotyledonary stages, better than on solid MGM culture medium. Therefore, regarding the production of synthetic seeds, results pointed out to the use of conventional techniques of encapsulation, but, with the addition of DEV nutritive solution on the encapsulating matrix for a conversion rate of synthetic seeds on seedlings of 77.5%.