Atividade de β-glucano e glutamina na integridade de mucosa e trofismo intestinal em camundongos Balb/C

Abstract

The aim of this study was to investigate the effects of β-glucan from yeast (Saccharomyces cerevisiae) on mucosal integrity, using a commercial product (Macrogard® - Biorigin) and the trophic effects of glutamine in the intestines of mice by inducing injury in intestinal tissue by administration of Ara-C. In the experiment we used Balb /C mice that were kept under controlled conditions of temperature and luminosity, receiving commercial food and filtered water ad libitum. The animals were divided into 8 groups and received treatment with the β-glucan (80 mg/kg) and / or glutamine (150 mg/kg), or both in combination, daily for a period of 21 days. In the last two days of this period, Ara-C was administered at a dose of 15 mg/kg) intraperitoneally every 12 hours. Analyzes were performed to evaluate the systemic toxicity on white cells blood and local gut-associated lymphoid tissue throught histopathological analysis and measurement of enzymes. The intestinal tropism was assessed by morphometry and mitotic index of the intestinal mucosa. We also evaluate the ability of β-glucan, with or without glutamine to reduce the toxicity of Ara-C on the mucosa by DNA test comet. Treatment with β-glucan was able to reverse the genotoxicity induced by Ara-C, to recover the parameters of intestinal structure, causing also mild hyperplasia of Peyer's patches and improving the general appearance of the tissue architecture of the intestine. Glutamine alone regained the height of the villi and the mitotic index of intestinal crypts and in combination with β-glucan was able to reverse the leukopenia. In conclusion, the use of β-glucan and glutamine alone reflects beneficial effects on intestinal trophism, but its effects in combination have yet to be clarified and β-glucan was effective in reversing some of the toxic effects induced by Ara-C.