Prospecção de genes tecido específico e metabólitos em Elaeis spp.

Abstract

The aim of this study was the exploration and validation of genes that have expression in specific tissue (leaf) present in the species Elaeis oleifera (Kunth) Cortés (caiaué) genome. Also, the development of an efficient protocol that enables the identification of secondary metabolites in leaves of the species Elaeis guineensis (oil palm) with the use of mass spectrometry coupled to liquid chromatography ultra high efficiency (UHPLC-MS). To step prospecting searches in genomic databases for genes that showed specific expression in leaf data were performed. After aligning the sequences of the candidate genes in the genome caiaué be performed, the results were validated using the technique of polymerase chain reaction in real time (qPCR). Two tissues (root and leaf) to compare differences in expression were tested. As a result was obtained indicating highest relative MyBHV5 gene expression in leaf tissue compared to root tissue and confirmation of the presence of this gene in the genome caiaué. While using the UHPLC-MS technique was possible to develop a protocol for extraction of secondary metabolites in leaves of palm oil which is efficient, simple and with good repeatability. It was possible to identify secondary metabolites which must be performed structural elucidation in future studies.