Phenotypic plasticity of leaf anatomical traits helps to explain gas-exchange response to water shortage in grasses of different photosynthetic types

Abstract

C3 and C4 plants, as their intermediates, respond differently to short-term changes in environmental conditions. This difference is linked to contrasting levels of phenotypic plasticity and photosynthetic apparatus specialization. Phenotypic plasticity is an underexplored topic although its understanding is crucial to predict plant behaviour in future climatic scenarios. In this research, the phenotypic plasticity of anatomical traits and its influence to carbon uptake efficiency was studied in plants with different photosynthetic types, under contrasting water regimes. Oryza sativa cvs. Soberana (drought-sensitive) and Douradão (drought-tolerant) (C3), Homolepis isocalycia (C3 proto-Kranz) and Andropogon gayanus (C4), grown at three water treatments (100, 75 and 50% of substrate water holding capacity), were phenotyped for leaf anatomy and gas-exchange parameters. The results showed that plasticity trends indicated different strategies between O. sativa cultivars to deal with water shortage, explaining their classification as drought-sensitive or tolerant. We also mapped typical characteristics of C3–C4 intermediate plant, H. isocalycia, mainly in the ratio mesophyll:bundle sheath cells and hypothesize how it may influence photosynthesis. Finally, we have confirmed previous claims that C4 carbon uptake advantages may be limited under severe drought conditions, as A. gayanus have drastically reduced its photosynthetic rates at lower water levels. By studying C3–C4 intermediates, this study may also be a starting point to unravel the trade-offs of anatomical changes during the evolutionary process from C3 to C4 photosynthesis, and also improve the understanding of their impact in carbon uptake in different water conditions.