Avaliação da resposta imunologica e trofismointestinal de camundongos tratados com β-Glucano e glutamina após desafio com ARA-C

Abstract

Systemic changes can predispose the increase of intestinal permeability, making possible the migration of bacteria and their products from the gut lumen into the sterile sites, leading to a local inflammatory process. Recently, Glutamine and β-glucan, have been demonstrated to play an important role in modulation of immune system and in promotion of intestinal health benefits. The aim of this study was to investigate the effect on inflammatory responses and intestinal health after the mice being pretreated with oral administration of soluble Saccharomyces cerevisiae, derived β 1,3/1,6, β-glucan (80 mg / kg) with or without glutamine (150mg/kg) and then challenged with administration of cytarabine (Ara-C) (15 mg / kg). The measured variables were: total of leukocytes, IL- 1β, IL-10 and INF-γ, intestinal morphology and immunohistochemical analysis of macrophage and lymphocytes T and B. Improvement in villi and crypts were not observed in β- glucan group, however, lower values of IL-10 and IL-1 β secretion were observed, whereas they prompt the highest release of IFN-γ. In mice treated with Glutamine was showed the intermediate value of all cytokines measured. The intestinal morphometric showed the best results in this group. The treatment of β-glucan in combination with glutamine presented the highest values of IL-1β and IL-10 and lowest values of the leukocytes total and INF-γ. The intestinal morphometric of this group showed the benefits on intestinal health. Based on these results, the answers of pretreatment with the combination of β-Glucan and Glutamine were reduction of intestinal inflammation and improvement of immune response after challenged with Ara-C.