In vitro establishment and multiplication of Echynochloa polystachy

Abstract

The objective of this work was to carry out the in vitroestablishment of Echynochloa polystachyaaiming at obtaining a micropropagation protocol for works involving the selection of superior genotypes and the cultivation of the species. E. polystachyastems were collected in the municipality of Manaus-AM. Explants were inoculated in test tubes containing Murashige and Skoog (MS) medium. Thirty days after in vitroestablishment, the rate of sprouting and contamination were evaluated. Experiments were also carried out to assess the effects of sucrose and 6-benzylaminopurine (BAP) concentrations on the tillering rate of explants. It was found that during the successive subcultures there was a decrease in internodes and the consequent loss of vigor. There were responses in the multiplication rate at concentrations starting from 45 g L-1sucrose.In addition, BAP and sucrose interfered the development and in vitromultiplication. Sucrose in conjunction with BAP was harmful and shortened internodes. The physiological state of the explants for the species under study was intrinsically linked to the concentrations of sucrose used for the culture medium and the concentrations of BAP. However, the sucrose and BAP concentrations suggested for in vitrocultivation of E. Polystachyamust be adjusted during successive subcultures. Absence of contamination in the in vitroestablishment occurred at concentrations 15, 30 and 60 g L-1sucrose. The combination of 1.5 mg L-1BAP and 30 g L-1sucrose promoted greater induction of sprouts. In addition, the in vitrorooting of E. polystachyawas 45%.