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In silico and quantitative analyses of the putative FLC-like homologue in coffee (Coffea arabica L.)
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Springer
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The sequential pattern of coffee flowering is a
major constraint that directly affects productivity, increases
harvest costs, and generates a final product of lower quality
for mixing dry fruits with ripe and unripe ones. The
objective of this work was to identify and analyze one of
the main genes involved in flowering regulation, FLOWERING
LOCUS C (FLC) in coffee (Coffea arabica L.). The
identification of this gene was conducted in silico using a
coffee EST database (CAFEST) and bioinformatics tools.
Quantitative PCR results suggest that the identified CaFLClike
homologue is directly involved in flowering regulation
in coffee. This expands our knowledge on evolutionary
conservation of flowering pathways in dicot species. The
functional studies of CaFLC-like with mutants of a more
tractable species will lead to a better understanding of the
molecular regulation as well as the specific functions of
each gene flowering during floral induction in coffee.
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BARRETO, H. G. et al. In silico and quantitative analyses of the putative FLC-like homologue in coffee (Coffea arabica L.). Plant Molecular Biology Reporter, Athens, v. 30, n. 1, p. 29-35, Feb. 2012.