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metadata.artigo.dc.title: Preparation of an affinity cryogel column for lysozyme purification
metadata.artigo.dc.creator: Veríssimo, Lizzy Ayra Alcântara
Paganoto, Fernanda Spagnol
Mol, Paula Chequer Gouveia
Fontan, Rafael da Costa Ilhéu
Minim, Valéria Paula Rodrigues
Minim, Luis Antonio
metadata.artigo.dc.subject: Lysozyme
Affinity chromatography
Cromatografia de afinidade
metadata.artigo.dc.publisher: Taylor & Francis 2017
metadata.artigo.dc.identifier.citation: VERÍSSIMO, L. A. A. et al. Preparation of an affinity cryogel column for lysozyme purification. Separation Science and Technology, New York, v. 52, n. 12, p. 1973-1982, 2017.
metadata.artigo.dc.description.abstract: Affinity cryogels were synthesized using tris(hydroxymethyl)aminomethane (Tris) as ligand for specific interactions with the lysozyme (LYZ). The cryogel was produced by cryo-copolymerization at −12°C. A central composite rotational design 22 was used to optimize the immobilization procedure of the Tris on the cryogel. A maximum adsorption for LYZ (149.5 mg/g) was achieved when 376 mg/mL of Tris and 3.06 mol/L of sodium borohydride were used during the Tris immobilization. Chromatographic separation of LYZ from chicken egg white was done with a purity of 92.13%. Results showed that the affinity cryogel was a potential separation medium for LYZ purification.
metadata.artigo.dc.language: en_US
Appears in Collections:DCA - Artigos publicados em periódicos

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