Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/31715
Título : Preparation of an affinity cryogel column for lysozyme purification
Autor: Veríssimo, Lizzy Ayra Alcântara
Paganoto, Fernanda Spagnol
Mol, Paula Chequer Gouveia
Fontan, Rafael da Costa Ilhéu
Minim, Valéria Paula Rodrigues
Minim, Luis Antonio
Palavras-chave: Lysozyme
Cryogel
Affinity chromatography
Lisosomos
Cromatografia de afinidade
Publicador: Taylor & Francis
Data da publicação: 2017
Referência: VERÍSSIMO, L. A. A. et al. Preparation of an affinity cryogel column for lysozyme purification. Separation Science and Technology, New York, v. 52, n. 12, p. 1973-1982, 2017.
Abstract: Affinity cryogels were synthesized using tris(hydroxymethyl)aminomethane (Tris) as ligand for specific interactions with the lysozyme (LYZ). The cryogel was produced by cryo-copolymerization at −12°C. A central composite rotational design 22 was used to optimize the immobilization procedure of the Tris on the cryogel. A maximum adsorption for LYZ (149.5 mg/g) was achieved when 376 mg/mL of Tris and 3.06 mol/L of sodium borohydride were used during the Tris immobilization. Chromatographic separation of LYZ from chicken egg white was done with a purity of 92.13%. Results showed that the affinity cryogel was a potential separation medium for LYZ purification.
URI: https://www.tandfonline.com/doi/abs/10.1080/01496395.2017.1318921?journalCode=lsst20
http://repositorio.ufla.br/jspui/handle/1/31715
Idioma: en_US
Aparece nas coleções:DCA - Artigos publicados em periódicos

Arquivos associados a este item:
Não existem arquivos associados a este item.


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.