Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/39224
Title: α-tocopherol encapsulation in ora-pro-nobis (Pereskia aculeata Miller) mucilage-whey protein isolate microparticles
Other Titles: Encapsulamento de α-tocoferol em micropartículas produzidas a partir de mucilage de ora-pro-nobis (Pereskia aculeata Miller) e isolado proteico de soro de leite
Authors: Resende, Jaime Vilela de
Veríssimo, Lizzy Ayra Alcântara
Rogers, Michael A.
Costa, Fabiano Freire
Carlos, Lanamar de Almeida
Veríssimo, Lizzy Ayra Alcântara
Silva, Vanelle Maria da
Keywords: Vitamin E
Canola oil
Coconut oil
Degradation kinetics
Isothermal behavior
Carrier oil
Bioaccessibility
Vitamina E
Óleo de canola
Óleo de coco
Cinética de degradação
Comportamento isotérmico
Óleo transportador
Bioacessibilidade
Issue Date: 6-Mar-2020
Publisher: Universidade Federal de Lavras
Citation: NEVES, I. C. O. α-tocopherol encapsulation in ora-pro-nobis (Pereskia aculeata Miller) mucilage-whey protein isolate microparticles. 2020. 85 p. Tese (Doutorado em Ciência dos Alimentos)–Universidade Federal de Lavras, Lavras, 2020.
Abstract: Microparticles of whey protein isolate (WPI) and ora-pro-nobis mucilage (OPN) encapsulated α-tocopherol were made using long-chain unsaturated (e.g., canola oil (CA)) or medium-chain saturated oil (e.g., coconut oil (CO)) as the carrier oil. Microparticleswere produced from CO- or CA-in-water emulsions by freeze-drying emulsions with various ratios of WPI/OPN. Before freeze dying, emulsions exhibited Newtonian or shear-thinning behavior. Smaller values of mean droplet diameter (230.37 nm) and polydispersityindex (0.144), and higher zeta potential modulus (-49.63 mV) were found for treatments with lower OPN content and prepared with CO. Drying yields for freeze-dried emulsions ranged between 74.1% to 87.1% w/w, depending on the biopolymers ratio and varied depending on whether CA or CO was used as the carrier. WPI:OPN ratios (between 23:1 and 7:1) nor oil phase (CO or CA) significantly affected the physical properties (e.g., oil retention, water content, and activity) of the dried powder between treatments. Higher powder bulk density (0.22 g•cm-3) and encapsulation efficiency (79.8% w/w) were obtained from freeze-drying CO,comparedtoCA-in-water emulsions and with higher concentrations of OPN. The surface roughness of the microparticles increased with increments in OPN concentration, regardless the carrier oil used, as observed by scanning electron microscopy.Over 35 days, α-tocopherol retention and degradation kinetics differed between CO and CA and was dependent on relative humidity. Absorbance peaks characteristic of groups found in the structure of proteins, carbohydrates, α-tocopherol and lipids (CA and CO) were observed in the infrared spectroscopy spectra. Bioaccessibility of encapsulated α-tocopherol was higher with CA WPI/OPN (23:1) (55.0±1.89%) compared to CO WPI/OPN (23:1) (42.4±1.78%), while the rate of α-tocopherol release and induction time for release were statically equal. It is concluded that the CO WPI:OPN(23:1) treatment was the one that presented the best performance for a possible industrial application, since it maintained high bioactive retention during the storage stability test and high bioaccessibility.
URI: http://repositorio.ufla.br/jspui/handle/1/39224
Appears in Collections:Ciência dos Alimentos - Doutorado (Teses)



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.